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ORIGINAL ARTICLE
Year : 2017  |  Volume : 4  |  Issue : 1  |  Page : 17-22

Effect of histone deacytylase inhibitors on allergic airway inflammation in mouse model anesthetized with ketamine


1 Department of Clinical Pharmacology, Faculty of Medicine, University of Alexandria, Alexandria, Egypt
2 Department of Clinical and Chemical Pathology, Faculty of Medicine, University of Alexandria, Alexandria, Egypt
3 Department of Pathology, Faculty of Medicine, University of Alexandria, Alexandria, Egypt
4 Department of Clinical Pharmacology, Faculty of Medicine, University of Alexandria, Alexandria; Wageh basha, Ganakls, Alexandria, Egypt

Correspondence Address:
Esraa Saeed Shaban Habiba
5A Wagehbasha, Ganakles, 1straml, Alexandria, 21532
Egypt
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2356-9115.202694

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Background Asthma is a chronic inflammatory disorder of the airways in which many cells and cellular elements play a role. The chronic inflammation is associated with airway hyper-responsiveness that leads to recurrent episodes of wheezing, breathlessness, chest tightness, and coughing, particularly at night or in the early morning. These episodes are usually associated with variable airflow obstruction within the lung that is often reversible either spontaneously or with treatment. Objective The present study was designed to examine the modulatory effects of the histone deacytelase inhibitor [valproic acid (VPA)] on ovalbumin (OVA)-induced airway inflammation in mice. Methods Airway inflammation was induced by means of intraperitoneal injection of 83.33 μg of OVA in 2.92 mg of alum on days 1, 11, and 14 and then challenged with 1% w/v OVA aerosol in PBS on days 15–18. However, normal control mice received PBS instead. The animals were then divided into three groups: the normal group, the untreated asthmatic group, and the VPA-treated group, which received 300 mg/kg of VPA on days 15–18 1 h before each nebulization. At the end of the experimental period (day 19), each animal was anesthetized with 80 mg/kg of ketamine intraperitoneally and then bronchoalveolar lavage fluid was collected and centrifuged. The collected supernatants of bronchoalveolar lavage were used to assess interleukin-4, whereas the cell pellets were collected and resuspended for cytological examination. Results Treatment with VPA resulted in a significant reduction in interleukin-4 level, total cell count, and neutrophil percentage in bronchoalveolar lavage fluid (P<0.05). The mean±SD was 16.55±1.07, 38.93±6.46, and 30.32±4.61 in the VPA-treated group versus 24.86±3.06, 99.03±6.67, and 70.40±8.30 in the untreated asthmatic group, respectively. Conclusion The treatment with VPA reduces inflammatory cellular infiltration, mainly neutrophils, in a mouse model of asthma.


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